After 24 h of culture in OCM, the phosphorylation of AMPK at Thr172 was reduced and was inversely correlated with the increased phosphorylation of mTOR at Ser2448 and phospho-pP70S6K Thr389 in ES-2 and SKOV3 ovarian cancer cells after culturing in OCM for 24 h (Fig. 5a). Here, PRKAA1 is linked to ovarian cancer.