A variability of 97%, 92%, 93%, 79%, 86%, 89%, and 82% for IP, LPS, LBP, HCC, IL-6, TNF-α, and body weight, respectively, were explained by the combination of key microbes and metabolites (Fig. 5f, Supplementary Data 1) altered by the tissue n-6/n-3 PUFA ratio. This evidence concerns the gene IL6 and hepatocellular carcinoma.