After 8 weeks of HFD, both sexes of 14 week-old HFD-Hhip+/+ mice developed a similar pattern of glucose intolerance (male, Fig. 2a; female, Fig. 2c), but hyperinsulinaemia was observed only in male HFD mice (Hhip+/+ and Hhip+/−) (Fig. 2b), not in female HFD mice (Hhip+/+ and Hhip+/−) (Fig. 2d), documented by plasma insulin levels measured during intraperitoneal glucose tolerance test (ipGTT). This evidence concerns the gene INS and Glucose intolerance.