Notably, mice with AP5/DNQX infusion to the LSv displayed reduced anxiety, spending more time in open arms in elevated plus maze tests (Fig. 4i) and more time in the center of recording arenas in open-field tests (Supplementary Fig. S5), suggesting that blocking ongoing glutamatergic action in LSv neurons reduces anxiety-like behavior, consistent with an increased anxiety level by photostimulating the PVH→LSv circuit. The gene discussed is AP5B1; the disease is Anxiety.