In Olsen’s study, they provided a global landscape of nonhistone protein methylation in esophageal squamous cell carcinoma (ESCC) cell with SMYD2 overexpression by using stable isotopic labeling with amino acids in cell culture (SILAC) coupled with immunoaffinity enrichment of monomethyl-lysine (Kme1) peptides and mass spectrometry [33]. The gene discussed is SMYD2; the disease is esophageal squamous cell carcinoma.