To investigate the biological function of RUNX1 in CRC cells in vitro and in vivo, we established stable RUNX1-overexpressing CRC cell lines with HCT116 and RKO cells and RUNX1-silenced cell lines using a lentiviral vector carrying two specific shRNAs in SW480 and RKO cells. This evidence concerns the gene RUNX1 and colorectal carcinoma.