CX3CL1 and neoplasm: In contrast, overexpression of miR-561-5p markedly reduced CX3CL1 expression in HepG2 and PLC/PRF/5 cells (Figure 3B).We also observed that basal levels of CX3CL1 negatively correlated with miR-561-5p levels in eight HCC cell lines (Figure 3C).Moreover, the lowest levels of CX3CL1 expression were observed in HCC with metastasis, whereas the highest levels were detected in the paired non-tumor tissues (Figure 3D-E).