In this study, liver markers (AFP, Hepatocyte, Glypican-3, and GS) and colon markers (CK20, CAD17, CDX2, β-catenin, and SATB2) were used for staining of xenograft-derived tumours, with the result that all markers were either expressed only weakly, or were not express at all. This evidence concerns the gene GPC3 and neoplasm.