To test whether the classical Hedgehog-GLI signaling pathway, in addition to the non-classical pathway Integrin αvβ3/ERK1/2, regulates GLI1 in gastric cancer MCAs, we pretreated BGC823 MCAs and SGC7901 MCAs with Integrin αvβ3 co-stimulator ligand RGD. This evidence concerns the gene MAPK3 and gastric cancer.