In a third study, Quigley and coworkers have performed whole genome sequencing of 101 samples of metastatic, castration-resistant prostate cancer tissue showing structural alterations of critical regulators of tumorigenesis not detectable by exome sequencing; the most frequent alteration consisted in the amplification of an intergenic enhancer region 624 kb upstream of the AR in 81% of patients, correlating with AR expression [68]. This evidence concerns the gene AR and Familial prostate cancer.