A third approach for the identification of prostate cancer stem cells consisted in the use of prostate tumor xenografts: using these cells in a series of studies Patra Wala et al. isolated CD44+/α2β1high cells from xenografts, and showed that these cells form tumor spheres in vitro and induce tumor formation following subcutaneous or orthoptic injection in immunodeficient mice [533,534,535]. This evidence concerns the gene CD44 and Familial prostate cancer.