LCN2 and alkaline phosphatase measurement: Serum NGAL, as measured by Sporek et al. [52] at 24, 48, and 72 h from the onset of AP, increased between the first and second day of AP, which was reflected by higher cut-off value to differentiate between mild and moderately severe/severe AP at 48 h (183 μg/L versus 165 μg/L at 24 h) (Table 4).