MYCN and neuroblastoma: More than 20 years ago, FISH was described as an alternative method to detect MYCN gene amplification in NB in place of Southern blot analysis.7 Subsequently, the presence of MYCN gene amplification was recognized as being associated with an aggressive clinical course in patients with NB8 and therefore required for the risk classification and treatment selection of these patients.1 Still, the availability of FISH assay remains generally restricted to developed countries.