To validate the findings from our ChIP-seq analysis, ChIP assays were performed, and the data showed that KIT was bound to the NFKBIB promoter in all three GIST cell lines, and KIT binding to the NFKBIB promoter was reduced in KIT-silenced GIST48 and GIST430 cells (Fig. 2b). This evidence concerns the gene KIT and gastrointestinal stromal tumor.