To verify that TMER5-derived shRNAs could be functionally expressed in the context of virus infection, NIH 3T12 cells were infected with MHV68.EW.shR or MHV68.SC.shR, and the levels of endogenous EWSR1 mRNA and EWSR1 protein were quantified using qRT-PCR and Western blotting. The gene discussed is EWSR1; the disease is viral infectious disease.