Thus, 16 days after infection of wild-type B6 mice with control wild-type virus or viruses expressing scrambled or anti-EWSR1 shRNAs, splenocytes were harvested for B cell subset staining with CCF4-AM and with antibodies directed against the B cell surface markers CD19, GL7, and IgM (Fig. 7A). Here, CD40LG is linked to infection.