STAT3 and glioblastoma: We performed western blot analysis to confirm phosphorylated STAT3 Tyr705 (pSTAT3, Y705) was present in our cell lines; activated pSTAT3 was present at high basal level in both adult GBM and pHGG lines including dBT114 (adult GBM) and MC-PED8 (H3K27M pHGG) and MC-PED17 (H3K27M pHGG) cells (Fig 1D).