TGFB1 and breast cancer: We found that TGFβ signalling increased the scratch closure process as compared to that in the control well, whereas, rhPRG4 significantly delayed the scratch closure process in the absence or presence of TGFβ suggesting suppression of cell migration (Fig 2C and 2D). Collectively these results demonstrate that rhPRG4 potently inhibits the invasive and migratory properties of breast cancer cells in vitro.