Nevertheless, our data from the human PCa cell lines on the effect of MK-2206 in promoting EMT along with the observation of reduced phosphorylated S473Akt in 5+5 Gleason PCa samples compared to 3+3 Gleason PCa samples, a trend toward elevation of TGFβ1, N-cadherin and Snail mRNA levels in the distant metastatic tumors compared to PCa tumors from MSKCC/UMICH study and reduced phosphorylated S473Akt and T308Akt associated with increased TGFβ1 and N-cadherin mRNA levels in the TCGA study suggest a negative correlation between Akt activity and EMT/metastasis in human the advanced PCa. This evidence concerns the gene TGFB1 and metastatic neoplasm.