However, α-DR3 exacerbated the colitis indicated by above indexes in Rag1–/–Rorcgfp/+, but not in Rag1–/–Rorcgfp/gfp mice, suggesting ILC3s are required for the pathogenic effect of α-DR3 in DSS-induced innate colitis (Fig. 7n–q; Supplementary Fig. 8I, J). This evidence concerns the gene RAG1 and colitis.