Quantification of the bands from the immunoblots defined higher FREM2 expression levels in glioblastomas for GBM versus REF (****, p < 0.0001) and GBM versus LGG (***, p = 0.0009), as shown in Figure 1C. Differences in FREM2 expression levels were supported also by the ELISA experiment, for GBM versus REF (p < 0.0001) and GBM versus LGG (p = 0.0032) (Figure 2). This evidence concerns the gene FREM2 and glioblastoma.