Cytoskeleton-associated proteins-enriched exosomes from metastatic colon cancer cells affected the integrity of endothelial cells by inducing a clear cytosolic delocalization of the adherens junction proteins including β-catenin, p120-catenin, and VE-cadherin, without changes of VE-cadherin expression at both mRNA and protein levels, through RhoA/Rock signaling in recipient cells. Here, CDH5 is linked to malignant colon neoplasm.