CD8A and infection: In addition to cytokine production, we evaluated the cytotoxicity of these TEWETGQI-specific CD8+ T-cells and during infection these cells show high cytotoxicity [32]; therefore, we decided to perform the analyzes in parasite-specific CD8+ T-cells generated only by immunization because during the infection the cells are very cytotoxic and it is difficult observed differences among the groups [3].