Although previous studies have shown CXCR3 role in the migration of effector lymphocytes involved in the control of viral, protozoan and bacterial infection [49–60], our study reveals for the first time that CXCR3 receptor is pivotal for the migration and positioning of pathogen-specific CD8+ T-cells directly involved in the clearance of T. cruzi after the prime-boost immunization and challenge. Here, CXCR3 is linked to bacterial infectious disease.