CD163 and neoplasm: The maker gene of M2 macrophage was selected from the prior publications.25, 26 The tumor purity was negatively linked with the expression of CD163, VSIG4, MS4A4A, MRC1, CCL24, and CD209, indicating that the result is reliable, because the higher tumor purity represented a lower proportion of TIICs, including M2 macrophages.