To investigate the expression of FLRT3/LPHN/Tim-3/galectin-9 pathway components in cancer cells of different origins, we screened various human cancer cell lines (derived from brain, colorectal, kidney, blood/mast cell, liver, breast, prostate, lung, and skin tumors) and various non-malignant cell lines and primary cells, using Western blot analysis. Here, LGALS9 is linked to cancer.