We showed the negative correlation between DKK4, derived from tumor cells, with the markers of CD8+ T cells, such as CD8, ZNF91, and SLC16A7 (Figure 5C), indicating that DKK4 overexpression decreased CD8+ T cells infiltrating into TME.40 What's more, interferon‐γ (IFN‐γ) and tumor necrosis factor (TNF), as typical effector cytokines of T cells, were standardized by T cells’ marker (CD3) for the assessment of cytotoxic immune cells activity, which showed consistent negative correlation with DKK4 expression (Figure 5D,E). The gene discussed is CD8A; the disease is neoplasm.