Heatmap plot shows that frequently mutated genes such as TP53 gene, which tends to been enriched in high TMB group (top 30 samples, range: 139–465 NOMs) in lung cancer; EGFR mutants in low TMB (bottom 30 samples, range: 37–82 NOMs), and patients with co-existence of TP53 and EGFR mutations in the intermediate TMB level (median 29 samples, range: 83–136 NOMs) (Fig. 4a). This evidence concerns the gene EGFR and lung cancer.