Subcellular fractionation assays showed that Lnc34a was mainly in the nuclear of HCC-LM3 cell (Fig. 1b) and SMMC-7721 cell (Fig. 1c), and RNA fluorescence in situ hybridization (RNA FISH) also suggest that Lnc34a was primarily located in the nucleus (Fig. 1d); thus, Lnc34a may function via epigenetic regulation. This evidence concerns the gene MIR34AHG and hepatocellular carcinoma.