SMAD4 and hepatocellular carcinoma: qRT-PCR (C and D), ELISA (E), Western blot (F), wound-healing assay (G; Magnification × 40) and transwell assay (Magnification: × 100) without (H) or with (I) Matrigel and CCK-8 assay (J), were conducted in SMMC-7721 cells transfected with miR-34a kd, shSmad4, miR-34a kd plus shSmad4 or NC, and in HCC-LM3 cells transfected with miR-34a, Smad4, miR-34a plus Smad4 or NC, induced with TGF-β1.