CASP3 and melanoma: In support of previous reports, silencing MYO1B, which augmented nuclear PTEN level, induced cell apoptosis of MEFs and melanoma cell B16F10 as reflected by enhanced Annexin-V immunostaining (Figures 5A, 5B, 6A, and 6B) and increased cleaved caspase 3 level without a change in PCNA level (Figures 5C, 5D, 6C, and 6D).