FN1 and chronic kidney disease: Moreover, microscopic histologic signs of CKD transition, including tubular atrophy, interstitial fibrosis and inflammation, were abrogated after lithium treatment, as demonstrated by immunoblot analysis of kidney homogenates for fibronectin and collagen I (Fig. 7F) and verified by Masson trichrome staining and by immunohistochemistry staining for fibronection (Fig. 7G).