Similarly, in the NSCLC tissues of patients with positive PD-L1 expression in CTCs, the mean value of PD-L1 expression detected by IHC was 33.7%, significantly higher than that of the negative PD-L1 expression (mean value of 24.5%) group in CTCs (P = 0.0313; Fig 6D). This evidence concerns the gene CD274 and non-small cell lung carcinoma.