WEE1 and infection: To dissect the host biochemical pathways involved in S. aureus cell invasion, HMEECs were pretreated with actin polymerization inhibitor, cytochalasin D, or microtubule disrupting agent, nocodazole, colchicine and vinblastine, protein kinase inhibitor, staurosporine as well as three inhibitors of cholesterol metabolism, methyl-β-cyclodextrin (MβCD), nystatin and filipin, followed by infection with bacteria.