Finally, to test the safety and tolerability of TKM-080301 in subjects with advanced hepatocellular carcinoma and to find the maximum tolerated dose (MTD) providing a preliminary assessment of anti-tumor activity of TKM-080301, an extended phase I/II TKM-PLK1 clinical trial for HCC was done (NCT 02191878, 2014, “Open-Label, Multi-Center, Phase 1, Dose Escalation Study With Phase 2 Expansion Cohort to Determine the Safety, Pharmacokinetics and Preliminary Anti-Tumor Activity of Intravenous TKM-080301 in Subjects With Advanced Hepatocellular Carcinoma”). This evidence concerns the gene PLK1 and hepatocellular carcinoma.