ABCC8 and pancreatic insulinoma: For structure determination, a FLAG-tagged hamster SUR1 and a rat Kir6.2 (95% and 96% identical to human, respectively) were overexpressed in the insulinoma cell line INS-1, and the channel complex affinity purified via the FLAG-epitope tag as described for our recent cryoEM structure determination of KATP bound to GBC and ATP (Martin et al., 2017a; Martin et al., 2017b).