INTS6 and cancer: For all identified essential genes except FAM22A, we observed a correlation between DNA copy number and transcript level across 947 human cancer cell lines from the CCLE23 (Supplementary Fig. 9), and data from pooled CRISPR/Cas920,38 and shRNA knockdown screens2,5,47 indicate that hemizygous deletion of HAUS6, RPS6, SMU1, NOL6, RPP30, MED4, INTS6, and ELAC2 increases the sensitivity to further knockdown of these genes (Supplementary Table 2).