SIRT1 and status epilepticus: To demonstrate the temporal change of mitochondrial biogenesis machinery expression following prolonged seizures, we showed NRF1 expression in the total protein prepared from the right hippocampal CA3 subfield, which revealed a significant increase in the expression of SIRT1 from 3 to 24 h with a peaking level at 6 h after KA-induced experimental status epilepticus (Figure 2A).