SIRT1 and status epilepticus: For conforming the specific effect on SIRT1 expression, after a pre-treated microinjection with antisense ODN for Sirt1 (1 nmol) into the CA3 subfield, real-time PCR showed a significant decrease in the Sirt1 mRNA level (Figure 3A), and Western blot analysis revealed a reduction in the SIRT1 protein level in the hippocampal CA3 subfield 3 h after experimental status epilepticus (Figure 3B) when compared with the control groups of sense ODN or scrambled ODN.