Although Su et al showed that a single dose of ethyl pyruvate (100 mg/kg) decreased NF‐κB and plasma cytokines in 3 h, but those inflammatory parameters were elevated in 9 hours in high‐dose LPS (30 mg/kg)‐challenged mice for survival test.55 In the subsequent study, ethyl pyruvate at different doses of 100 mg/kg or 50 mg/kg was proven to reduce the mortality from endotoxin‐induced ALI and the permeability index in mice.34 Notably, the beneficial effects of 100 mg/kg ethyl pyruvate were superior compared to those of 50 mg/kg, which are consistent with our dose‐response results (Figure S1). Here, NFKB1 is linked to acute respiratory distress syndrome.