The protein expression levels of Copine 6 and p-catenin/catenin was decreased (P < 0.05 or P < 0.01), whereas the protein expression level of p-GSK3β/GSK3β was increased (P < 0.05 or P < 0.01) in the hippocampus and PFC of NAFLD rats. This evidence concerns the gene GSK3B and metabolic dysfunction-associated steatotic liver disease.