To investigate the potential effect of RES on NAFLD and explore the possible mechanism, a NAFLD rat model was established using high-fat diet in the present study, and the protein expression levels of p-GSK3β/GSK3β, p-catenin/catenin, cyclin D, and Copine 6 in the hippocampus and PFC were measured using Western blotting. This evidence concerns the gene CPNE6 and metabolic dysfunction-associated steatotic liver disease.