HT-29 human colorectal cancer cells were transfected with vectors encoding the wild-type (SDC2) or mutant versions (D49A, Y51A) of syndecan-2, and then co-immunostained using antibodies against syndecan-2 and MMP-7 followed by Texas Red- and fluorescein isothiocyanate-conjugated secondary antibodies. The gene discussed is SDC2; the disease is colorectal cancer.