In esophageal squamous cell carcinoma (ESCC), the expression of PlncRNA-1 was significantly higher in human ESCC compared with the adjacent noncancerous tissues (69.8%, p < 0.05), and this observation correlated with advanced clinical stage (p < 0.01) and lymph node metastasis (p < 0.05). This evidence concerns the gene CBR3-AS1 and esophageal squamous cell carcinoma.