We found that leptin treatment significantly increased, at all times investigated, the expression of Tsg101, an important component of the endosomal sorting complex required for transport I (ESCRT-I) involved in the ESCRT-dependent mechanisms of exosome biogenesis [32], in both MCF-7 and MDA-MB-231 breast cancer cells (Figure 4A). This evidence concerns the gene TSG101 and breast cancer.