As shown in Figure 2, in the presence of LDFI peptide, leptin effects on MVB formation and exosome secretion were significantly reduced in both MCF-7 and MDA-MB-231 breast cancer cells, as evidenced by TEM analysis (Figure 2A), NTA (Figure 2B), as well as the measurement of acetylcholine esterase activity (AchE activity), an additional assay used to quantify exosomes (Figure 2C). This evidence concerns the gene LEP and breast cancer.