Mechanistically, we confirmed that elevation of Tsg101 protein expression as well as induction in the MVB formation and in the number of released exosomes observed in breast cancer cells treated with leptin are dependent on the chaperoning activity of Hsp90, since leptin effects were no longer detectable in the presence of the Hsp90 inhibitor 17-AAG. Here, TSG101 is linked to breast carcinoma.