Analysis of high-frequency mutated genes showed 12 known gene mutations in colon cancer-associated pathways, including APC, TP53, SOX9, TOPORS, IGF1, KLF5, LSR, CALM2, CTNNB1, RSPO2, SYF2, and SHISA4. The normal-colorectal adenoma somatic mutation analysis identified mutations in two key genes, APC and CTNNB1, which are known to be involved in the Wnt signaling pathway. Here, RSPO2 is linked to colorectal adenoma.