Importantly, the adoptive transfer of Gal-3−/− DCsLPS did not alter serum level of KYN and total number of colon-infiltrating Tregs in DSS-treated mice (Figure 6E,F), suggesting that Gal-3 was required for the optimal production of KYN in TLR-4-primed DCs and for consequent Treg-based alleviation of Th1/Th17 cell-driven colitis. This evidence concerns the gene TLR4 and colitis.