Further study reveals that primary CD34+ CML cells cultured with 13C 6-glucose significantly decrease the incorporation of 13C isotopes into citrate, glutamate and aspartate as well as the enzymatic activities of pyruvate dehydrogenase (PDH) and pyruvate carboxylase (PC) after tigecycline treatment, resulting in a robust decrease in glucose oxidation and extra cellular acidification [35]. This evidence concerns the gene PC and chronic myelogenous leukemia, BCR-ABL1 positive.