To precisely determine their B-cell phenotype and check their identity using flow cytometry after each thawing, we evaluated the expression of 27 markers routinely investigated in mature B cell lymphoma (membrane markers: Kappa, Lambda, CD19, CD20, CD22, CD23, CD5, CD10, CD27, CD38, CD39, CD43, CD62L, CD81, CD200, FMC7, IgM, LAIR1, CD123, CD103, CD25, CD11c, CD71, and CD180, and intra-cytoplasmic markers: Ki67, BCL2, and BCL6) (Supplementary Table S2). This evidence concerns the gene CD27 and B-cell non-Hodgkin lymphoma.