Double immunofluorescence stainings for PDPN and K15 confirmed the absence of PDPN expression in HF keratinocytes of K5-Cre;PDPNflox/flox mice, whereas PDPN was located in HF keratinocytes of littermate PDPNflox/flox control mice (Fig 2A). The gene discussed is KRT5; the disease is hydrops fetalis.