TSP1 inhibits stem cell self-renewal by downregulating the expression of self-renewal genes through its receptor CD47 in primary murine endothelial cells; decreases the expression of self-renewal genes and sphere-forming capacity in human colon cancer (HCT116), non-small cell lung cancer (A549), and cervical cancer (HeLa) cell lines; CD47, a TSP1 receptor, is highly expressed in circulating hematopoietic stem cells, leukemia cells, breast CSCs, pancreatic CSCs, and AML leukemia stem cells and required for self-renewal of these CSCs. This evidence concerns the gene THBS1 and leukemia.