As shown in Fig. 5A, an upregulation of CD69 activation marker occurs for both CD4+ (Fig. 5AI) and CD8+ (Fig. 5AII) UniCAR T cells in a strict tumor-specific and TM-dependent manner, while the activation level of UniCAR T cells redirected by the His-tagged or un-tagged TM was indistinguishable. Here, CD69 is linked to neoplasm.