We confirmed the effects of NanoFEN on cell cycle factors by validating the increase in total p16 levels and phospho-p38 MAPK, and the decrease in phospho-Aurora A-B-C and phospho-PLK1 by immunoblotting in NanoFEN-treated lung and CRC spheroids (Fig. 5b). The gene discussed is CDKN2A; the disease is colorectal carcinoma.