In order to determine the effect of PHGDH overexpression on follicular melanin throughout the HF cycle, two approaches were used: one set of mice was fed dox diet for 2 days before plucking (red bar) and the other set for 30 days before the HF cycle was synchronized by plucking (blue bar) (Fig. 2a). Here, PHGDH is linked to hydrops fetalis.