S100B and metastatic melanoma: Analysis of the specificity and sensitivity using the results obtained for samples from healthy donors (n = 28) and patients with metastatic melanoma (n = 29) tested with both assays, showed that sensitivity was 48% for S100-EPISPOT and 21% for CellSearch® (p = 0.0114, corrected p = 0.0269), and specificity was 100% for S100-EPISPOT and 96% for CellSearch® (Table 1b).