We identified differentially expressed microRNAs based on three criteria: 1) because KLF5 is a transcription factor, the selected differentially expressed microRNAs were downregulated in KLF5-knockdown cells; 2) differentially expressed microRNAs inhibited expression of tumor suppressors in the pancreatic cancer pathway extracted in KEGG; 3) interactions between selected differentially expressed microRNAs and target genes such as tumor suppressors and components of the pancreatic cancer pathway were experimentally verified using the miRTarBase (24304892) database. The gene discussed is KLF5; the disease is familial pancreatic carcinoma.